Photosynthesis - pigment factor - basic principle

Distribution of pigments in Coleus Area of the leaf with no pigments Area of the leaf with anthocyanin Area of the leaf with chlorophyll

Procedure to study pigments Note the color change in specific areas of the leaf Bleached leaf is placed in a petri dish and Lugol's is added to the dish Hot alcohol breaks down cell emmbranes, pigments leach out of the cells, and the leaf is bleached Boil the leaf in hot alcohol Note the different colored areas on a Coleus leaf

Result of pigment experiment Negative with Lugol's - absence of starch Positive with Lugol's - presence of starch Area of the leaf with no pigments Area of the leaf with anthocyanin Area of the leaf with chlorophyll