Basic principle of paper chromatography The pigments are separated on the basis of their molecular size, solubility in the solvent, and affinity to the paper As the solvent is absorbed by the paper, the pigment mixture travels with the rising solvent As the solvent is absorbed by the paper, the pigment mixture travels with the rising solvent The paper strip is then dipped into a solvent making sure that the pigment spot is above the level of the solvent An extract of photosynthetic pigments is prepared and applied on a chromatography paper strip to create a small concentrated spot

Pigment extraction Cut out a chromatography paper strip to the size of the test-tube; use a capillary tube to apply the pigment extract on the paper; repeat the applications till a dark concentrated spot is formed Grind the leaves to extract the pigments in acetone Add the leaves, sand and acetone to a mortar-pestle Take chopped green leaves, a pinch of sand, and about 7 mL of acetone

Pigment separation